Ion Exchange Chromatography

Most molecules have a net charge within a pH range of 2 to 10. When the pH is altered, the net charge on molecules can change drastically. In this experiment, a mixture of two chemicals is absorbed onto a solid support ion-exchange column and separated during elution under conditions that influence their net charge.

Kit includes: Instructions, ion exchanger, chemical mixture, potassium acetate buffer and chromatography columns.

All you need: spectrophotometer and cuvettes, ring stands and clamps, test tubes and 5ml pipettes and pumps.